A single intranasal dose of EV25, a bispecific small molecule developed by Eradivir, acts faster than the current standard of care, eliminating the detectable virus within 24 hours. EV25 also has a window of efficacy of 96 hours postinfection, which is broader than the current standard of care.

A research paper titled “Targeted recruitment of immune effector cells for rapid eradication of influenza virus infections” has been published in the peer-reviewed Proceedings of the National Academy of Sciences.

EV25 was built on a platform created by Philip Low, the Presidential Scholar for Drug Discovery and the Ralph C. Corley Distinguished Professor of Chemistry in Purdue University’s College of Science. He is part of Purdue’s One Health initiative and on the faculty of the Purdue Institute for Drug Discovery and the Purdue Institute for Cancer Research. Low is Eradivir’s chief scientific officer and on its board of directors.

He disclosed the immunological innovations to the Purdue Innovates Office of Technology Commercialization, which has applied for patents to protect the intellectual property. OTC licensed the innovations to Eradivir for further development and commercialization.

Serious threats posed by influenza

Imrul Shahriar, a scientist at Eradivir, said the influenza virus remains a serious health threat for multiple reasons. One is that current FDA-approved drugs are only effective when administered early during an influenza infection, but they are less effective when administered in later stages of the disease.

“This emphasizes the need for a therapy that can treat more severe infection,” he said. “Familiarity with influenza may mollify public concerns about its risk, but infections still cause tens of millions of illnesses and medical visits annually in the United States, hundreds of thousands of hospitalizations, and tens of thousands of deaths.”

Other reasons influenza remains a serious health problem are:

• Only 50% of people in the U.S. get vaccinated, and the effectiveness of current vaccines ranges from only 19% to 60%.
• Current therapies are less effective against some strains of the flu as they mutate.
• Pandemic strains of the flu like the current H5N1 avian strain may mutate enough to be transmitted from animals to people and eventually from people to people. They are now being transmitted to dairy cattle that, in turn, are infecting people.

Developing and testing EV25

Shahriar, who recently earned his PhD in Low’s laboratory, said EV25 reduces viral loads of advanced-stage influenza in two ways.

“It binds and inhibits viral neuraminidase expressed on both free virus particles and virus-infected cells,” he said. “It also recruits naturally occurring antibodies to fight the virus.”

Shahriar said tests showed EV25 lowered secretions of pro-inflammatory markers and protected against virus-induced damage to lungs better than current therapies.

“As a result, we believe that combining an immunotherapy with a chemotherapy in a single pharmacological agent constitutes a promising new approach for treating the more challenging forms of influenza virus infection,” he said.

Next development steps

Recently EV25 was approved by the European and Belgian regulatory agencies to be used in a Phase 1 human trial that will conclude early next year. Shortly following the Phase 1 trial, a Phase 2a trial will be conducted to further establish the safety and begin to define the efficacy of EV25. Data from the Phase 2a will be available in July 2025.

Eradivir at OPTIONS XII

Jeffery Nielsen, vice president for research and development at Eradivir, presented information on two Eradivir drug compounds during OPTIONS XII, a global scientific conference focused on influenza held Sept. 29-Oct. 2 in Brisbane, Australia.

The poster presentation “Ligand-Targeted Immunotherapy for the Rapid Clearance of Influenza Infections” disclosed data about EV25. It won the Best in Clinical Science and Vaccinology award at the conference.

The oral presentation “Novel Ligand-Targeted Immunotherapy for the Treatment of Human Respiratory Syncytial Virus (RSV)” disclosed data about Eradivir’s small-molecule therapy to treat human RSV infections in all age groups.

Both EV25 and the RSV therapeutic are built on the BAiT platform, or Bispecific Antigenic immunoTherapy, developed at Purdue.

“It was tremendous that presentations about both therapeutics were accepted at OPTIONS XII, the premier conference on influenza and respiratory viruses,” Nielsen said. “EV25 is a game changer in how effective it is compared to existing therapeutics in preclinical models. The RSV drug is another game changer because it rapidly clears out infection in preclinical models; there are currently no therapies to treat a person who has been infected with RSV.”

A new study reveals that piangua populations are showing concerning signs of decline, largely due to overharvesting. Researchers used cutting-edge DNA analysis to examine these shellfish in two key locations along Colombia’s Pacific coast, providing the first detailed genetic profile of these important creatures.

The findings paint a worrying picture. “While piangua populations have been stable for over 100,000 years, recent decades of intensive harvesting have taken their toll,” explained Silvia Restrepo, president of the Boyce Thompson Institute and lead author of the study.

The numbers are striking. Piangua exports from Colombia skyrocketed from just 100 tons per year in 1980 to over 3,000 tons by 2004. In some areas, like Bahía Málaga, the population has plunged by 60%. This dramatic increase in harvesting has forced the piangua onto Colombia’s endangered species list.

“We discovered that the piangua are experiencing a significant loss of genetic diversity,” said Restrepo. “This is evidenced by a high level of inbreeding and a reduction in heterozygosity.”

Why is genetic diversity so important? Imagine the gene pool of a species as a deck of cards. Each card represents different genetic traits that help the species survive various challenges — disease resistance, temperature tolerance, or the ability to find food. When overharvesting reduces the population, it’s like removing cards from the deck. With fewer “cards” in play, the species becomes less equipped to handle new threats.

The study, recently published in Scientific Reports, also revealed something unexpected. Despite living in connected coastal waters, piangua populations in different areas maintain subtle genetic variations. This suggests that local populations might have special adaptations to their specific environments, making it even more crucial to protect them all.

This research has real-world implications for conservation. Equipped with this new genetic information, scientists, the Colombian government, and local communities can work together to develop better protection strategies. These might include creating protected areas, establishing sustainable harvesting quotas, or implementing restoration programs.

The study marks a significant milestone as the first genetic investigation of piangua in Colombia, complete with the first detailed mapping of its genome. This genetic blueprint will be an invaluable tool for future conservation efforts.

The message is clear for the communities along Colombia’s Pacific coast: without immediate action to protect these vital shellfish, they risk losing not just an important food source, but a crucial part of their cultural and economic heritage. The race is on to save the piangua while there is still time.

This research was funded by the Ministerio de Ciencia, Tecnología e Innovación (MinCiencias) of Colombia.

Individuals relying on shallow groundwater or natural springs for drinking water have a higher risk of contamination from these insecticides compared to those getting their drinking water from deep groundwater wells. They also found that these insecticides could affect organisms in the streams that are fed by those springs.

The research was recently published in Science of the Total Environment, a peer-reviewed scientific journal.

With the increased use of insecticides for agriculture and insect control in the past few decades, research has shown that consistent application of them can lead to contamination in water. This can have a negative impact on human health and pollinator insect species, such as bees and butterflies.

A large portion of Minnesota’s drinking water comes from groundwater. Looking at the levels of these specific insecticides can help water treatment plants determine what to monitor and filter.

In this study, researchers looked at the levels of neonicotinoids in surface and groundwater across Minnesota, identifying factors that may affect the extent of contamination. The DNR was able to collect water samples from 15 springs and 75 unique wells from 13 counties in Minnesota over four years (2019-2022) allowing comparisons between urban and rural areas.

The researchers not only collected an extensive amount of geographic locations, they also looked at the chemical levels at various depths. In testing the water samples, they found that natural springs are very susceptible to contamination from neonicotinoid insecticides.

“Oftentimes people think a natural spring has water that is safe to drink,” said Bill Arnold, Distinguished McKnight University Professor & Joseph T. and Rose S. Ling Professor in the University of Minnesota’s Department of Civil, Environmental, and Geo- Engineering. “That’s not necessarily true, it depends on how deep that water is sourced.”

There are multiple ways these types of chemicals can get into the environment. In addition to direct application, stormwater runoff and snow melt can contribute to these chemicals in ground and surface water. The researchers were able to map different classes of the insecticides across the state.

“This study is a key first step in identifying pollutants in the water, even in some unexpected places like natural springs,” said Arnold. “We use so many of these insecticides in Minnesota — basically every corn and soybean seed is treated before planting — and many of these chemicals can be purchased at a local hardware store for spraying fruit trees, flea and tick control for your pets, and eliminating bedbugs.”

While the researchers knew they would find traces of the insecticide in water, some of their study results were unexpected.

“We were surprised by the high levels of the chemical detected in the natural springs and in some cases the chemicals were observed at much deeper levels in wells than we expected going into this project,” Arnold added.

As a follow-up to the study, researchers are collaborating with county and state agencies to include these chemicals on water treatment monitoring lists and want to continue to educate watershed districts about these insecticides. The researchers will also work with the University of Minnesota’s St. Anthony Falls Laboratory to improve stormwater treatment methods for urban environments, including the use of biofiltration to remove pollutants.

In addition to Arnold, the team included University of Minnesota Department of Civil, Environmental, and Geo- Engineering retired adjunct associate professor Paul Capel, former research assistant Grant Goedjen, and John Barry from the DNR’s Division of Ecological and Water Resources.

This work was funded by the Minnesota Environment and Natural Resources Trust Fund (ENTRF) as recommended by the Legislative Citizen Commission on Minnesota Resources (LCCMR). The ENRTF is a permanent fund in Minnesota that provides funding for the protection and conservation of Minnesota’s natural resources.